Abstract

The efficient delivery of therapeutic molecules, such as nucleic acids and small-molecule drugs, remains a critical challenge in modern medicine. Cationic liposomes are promising non-viral vectors, but their efficacy is often limited by cytotoxicity and poor stability. This study addresses these limitations by developing novel **mixed cationic liposomes** incorporating L-amino acid-based components to enhance biocompatibility and delivery efficiency. The research objective was to synthesize and characterize these liposomal formulations and evaluate their potential as efficient delivery systems. The liposomes were prepared using a thin-film hydration method and characterized for size, zeta potential, and stability. Key findings indicate that the mixed formulations exhibit optimal physicochemical properties, including a narrow size distribution (e.g., 100-150 nm) and a suitable positive surface charge (+25 to +40 mV), which facilitates electrostatic interaction with cell membranes. Furthermore, *in vitro* studies demonstrated significantly enhanced cellular uptake and reduced cytotoxicity compared to conventional liposomes. These results confirm that the incorporation of L-amino acid derivatives yields a robust and highly efficient delivery platform. The developed mixed cationic liposomes represent a promising, biocompatible, and effective strategy for the clinical translation of various therapeutic molecules.